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Bright-field light microscopy of material preserved in ethanol

Image 1: basidiospore in lateral perspective; basally hyaline and smooth, apically brown and echinulate; 8.9–⁠14.3 μm wide (x̄ = 12.0 ± 1.5 μm, n = 10)
Image 2: basidiospores in apical perspective
Image 3: hymenium showing hyaline cystidia and basidioles
Image 4: hyaline stipe hyphae showing clamp connection; clamp connections abundant
Image 5: crushed stipe showing hyaline hyphae, appearing orange en masse

Corresponding macromorphological observation: https://inaturalist.org/observations/180410419

Sapromamanita galerumgandalfi (ined.), aka Noddy Flycap (Amanita 2 Ridley). Photos taken by Wael Abbas and posted here as a record with his permission. Unfortunately, the Sydney herbarium said it was unimportant so it was not kept for scientific study.

this had the consistency of gel. I thought initally that it was a resin coming out of the tree but we saw more along the track that wasn't associated with a tree. we wondered if it was some sort of animal residue rather than plant.

Many of these in clumps around the base of Bunya pine. Did not observe these in May / June 2023. This comes following a few weeks of substantial rain and first was much more damp than in winter.

Microscopy images: first four are congo red stain at 1000x magnification. The last one is mounted in KOH and viewed at 400X magnification.

I have included pictures of a clean culture of this specimen grown in PDA following 5 months incubation at ambient lab temperature in the dark.

Last four photos are of the collected sample (fresh), and the dried sample submitted to BRI in April 2024.

Growing from wet soil with high clay content. Sclerotiums are dense and hard to the touch. Am considering slicing one to get a good view of the internals of the sclerotium.

I know lignosus is not known in Australia but i believe it is in New Guinea; possible extension of range? Or I may just be wrong, heh

@mattbarrett @petragloyn